Comparison of isolated outer hair cells from five mammalian species
نویسندگان
چکیده
منابع مشابه
Comparison of isolated outer hair cells from five mammalian species.
Live outer hair cells were isolated from guinea pig, chinchilla, rat, mouse, and gerbil. The organ of Corti from selected turns of the cochlea was briefly incubated with collagenase and outer hair cells were separated from the tissue by micromanipulation under microscopic observation. Morphological criteria for cell viability were: cylindrical cell shape without swelling or distortion of the me...
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The outer hair cell (OHC) from the organ of Corti plays a crucial role in hearing through its unique voltage-dependent mechanical responses. Furosemide, one of the loop diuretics, disrupts normal cochlear function. Here we report on direct effects of furosemide on OHC motility-related, voltage-dependent capacitance using the whole-cell patch-clamp technique. Extracellularly applied furosemide r...
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Outer hair cells were isolated from the guinea pig cochlea using a micromechanical non-enzymatic procedure. Depolarization of outer hair cells in the presence of 25-125 mM K+ was accompanied by a longitudinal contraction of the isolated cells. A decrease of [K+] to 5.4 mM interrupted contraction and induced a relaxation. Individual hair cells were able to undergo as many as 5 cycles of contract...
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ژورنال
عنوان ژورنال: Hearing Research
سال: 1987
ISSN: 0378-5955
DOI: 10.1016/0378-5955(87)90061-x